Aim To study the active constituents for the treatment of rheumatoid arthritis from the ethyl acetate extracts of the roots
of
Lasianthus acuminatissimus Merr.Methods Various chromatographic techniques were used to separate and purify the constituents.Their structures were established on the basis of 1D,2D NMR and HRMS spectroscopic analyses and their preliminary evaluation of anti-inflammation effect on the release of β-glucuronidase was carried out.Results Eight compounds were isolated and identified as
lasianthuslactone A(1),codonolactone(2),2,5-dimethoxy-1,4-benzoquinone(3),uncargenin A(4),nonadecyl alcohol(5),13-docosenoic acid(6),tetracosanoic acid(7) and β-sitosterol(8). Compound 3 showed a significant inhibitory effect on release of β-glucuronidase rat polymorphous nuclear leukocytes activated by platelet activating factor(PAF).Conclusion Compound 1 is a new one,the others were isolated from the plant for the first time and 3 is one of active anti-inflammation compound in the plant.