PURPOSE:To study the effects of pingyangmycin(PYM)on the proliferation and expressions of
adhesion molecules of human venous
malformations
endothelial cells(HVMECs).METHODS:The proliferation inhibition of PYM on HVMECs was measured by MTT assay.Expressions of
adhesion molecules were investigated by immunocytochemistry and cellular ELISA.SPSS 11.0 software package was used for one way ANOVA.RESULTS:The inhibition rates showed a dose-dependent and time-dependent effect.In the control group,no expressions of E-selectin(CD62E)and vascular cell adhesion molecule-1(CD106)were observed in HVMECs.10μg/ml and 100μg/ml PYM,which didn't induce expressions of CD62E and CD106,had significant inhibitory effects on HVMECs.In contrast,increased expression of CD62E was detected after HVMECs were exposed to 0.1-1μg/ml PYM and expression of CD106 was detected after HVMECs were exposed to 0.5-1μg/ml PYM.CONCLUSIONS:The inhibitory effects of PYM on HVMECs has a dose-dependent and time-dependent relation.Low concentrations of PYM have a mild inhibitory effect on cellular proliferation but can stimulate HVMECs to express CD62E and CD106,which may play some role in the inflammatory process of venous malformations treated by PYM.