ObjectiveTo explore the inhibitory effect of
Fuzheng Yiliu Granule (FYG) on growth and apoptosis of H_ 22 tumor cell, and
expressions of p53 and Caspase-3 gene. MethodsThe tumor inhibitory rate of FYG on H_ 22 tumor cell line was observed in vivo,
cell apoptosis rate and cell cycle were determined by flow cytometry (FCM), and expressions of wild type p53 and Capase-3 mRNA were determined by RT-PCR. ResultsFYG could inhibit the growth of H_ 22 tumor cell at the dose of 12g/kg, 24g/kg, the maximal inhibitory rate up to 51.24% (P<0.01). By FCM, it was shown that FYG could significantly enhance apoptosis rate of cell line H_ 22 , with the peak reached 15.84% (P<0.01),and cause the tumor cell cycle being blocked at G_0/G_1 phase, with decrease of cells in S phase. RT-PCR illustrated that FYG could significantly up-regulate the level of p53 and Caspase-3 mRNA expression. ConclusionFYG can significantly inhibit the growth of tumor cell in mice, its anti-tumor mechanisms may relate to the cell apoptosis induction, cell cycle regulation and wild type p53 and capase-3 gene expression enhancing.