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Shvoong Home>Medicine & Health>Purification and anti-cancer activity of ricin Summary

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Purification and anti-cancer activity of ricin

Article Abstract by: TsingHua     

Original Author: Journal of Zhejiang University(Medicine Sciences)
  • Summary rating: 3 stars (1 Ratings)
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  • words:300 
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This abstract was translated from 蓖麻毒素的提取及其抗肿瘤作用研究
Objective: To extract and purify ricin from castor beans and to evaluate its anti-cancer activity. Methods: Ricin
was purified from castor beans according the modified method of Nicolson and Blaustin.The lectins were extracted in 0.01 mol/L phosphate buffered saline and isolated in the 40% to 80% fraction of ammonium sulfate precipitation.The dialyzed fractionated preparation was applied with a Sepharose 4B column.Thelectins were eluted with a linear lactose gradient (0.01 mol/L ～0.5 mol/L).Ricin wasseparated from the ricinus agglutinin by gel filtration on a Sephadex G-100.MTT was applied to analyze the cytotoxicity with different dosage of ricin in different cancer cell lines. Results: There was no difference between the killing effect of normal cells and that of colon cancer cells by using thehigh dosage of ricin(5×10~(-8) mol/L~5×10~(-10) mol/L).However,the cytotoxicity was significant different in those cells with the low dosage of ricin(5×10~(-11) mol/L~5×10~(-13) mol/L).Meanwhile ricin had the similar cytotoxicity to leukemia cell K562 and colon cancer cell SW480. Conclusion: Ricin is able to kill tumor cells selectively at low concentration,but the selectivity does not appear at high concentrations.
Published: May 25, 2005
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