Vincristini sulfas (VCR) is an important common antitumor drug. The resistance to anticancer drug is the main cause of the chemotherapy failure. To screen the drugs which can reverse VCR resistance for VCR resistant cell strain, an analytical
method has been established for the determination of VCR concentration in tumor cells using
high performance liquid chromatography (HPLC). The stainless steel column was 25cm× 4.6 mm i.d. packed with totally porous, spherical silica particles (5μm). A solution of methanol and 0.02mol/L dipotassium hydrogen phosphate (80∶20, V/V ) adjusted to pH 6.6 with H 3PO 4 was employed as the mobile phase. The flow rate was 1mL/min.
Chromatography was performed with ultraviolet detector at 267nm. The method is simple, rapid and selective. Linear calibration curve for VCR was measured within the range of 10 to 200mg/L with correlation coefficient of 0.999 8. The lowest detection limit was 4mg/L tumor cells extract. The HPLC method described is suitable for clinical monitoring and pharmacokinetic study of VCR.