ffect of
fluoride on the lipid
fluidity of some
biomembranes of rats was determined by
fluorescence polarization technique using DPH and ANS as fluorescence probes in vitro and in vivo in order to further understand the mechanism of cellular effect and membrane toxicity of fluoride. When exposure doses of fluoride from 1 to 128 mmol/L, the fluorescence polarization of ANS in synaptic membrane was increased significantly with obvious sode effect relationship (r=0.85, P<0.05), but the P of DPH was not altered in vitro. In vivo, sodium fluoride at 20mg/kg·day was injected intraperitioneally for 50days, the results showed that the fluorescence polarization of DPH in synaptic membrane, liver cell and erythrocyte membranes were significantly elevated. Considering the chemical properties of fluoride and the results, it is likely that effect of fluoride on the lipid fluidity of biomembranes probably result from either the direct interaction with biomembrane surface structures, or interference with lipid metabolism of biomembrane.\ \