Aim To observe the effects of ouabain and
aconitine on APD and ion channels in isolated
guinea pig and rat
ventricular
myocytes; to elucidate the action mechanisms of these two drugs and set up new
arrhythmie models on cellular level. Methods In isolated
ventricular myocytes of guinea pig and rat, the
effects of ouabain and
aconitine on APD, I_(Ca-L), I_k,I_(to), and I_(kl) were observed using the whole cell patch clamp
technique. Results Ouabain (5 μmol·L~(-1)) obviously prolonged the APD_(90), increased I_(Ca-L), decreased I_k and
I_(kl) in guinea pig ventricular myocytes. Aconitine (1 μmol·L~(-1)) lengthened the APD_(90), increased I_(Ca-L), decreased I_(to) and increased I_(kl) in rat ventricular myoeytes. Conclusion The targets on ouabain- and aconitine-
induced arrbythmias included APD, I_(Ca-L), I_k, I_(to) and I_(kl). APD, I_(Ca-L), I_k and I_(to) must be the powerful ones,
both in arrhythmic and antiarrhythmic courses. The ouabain- and aeonitine- induced arrhythmic models on
cellular level were built to study the antiarrhythmie mechanisms of chemicals and evaluate new drugs. These two
new-type models in vitro were stable, liable, repeatable and economic, which were superior to those typical models in vivo.