ObjectiveTo investigate the effects of
carbachol injection in intestine on plasma levels of tumor necrosis factor-α(TNF-α),
interleukin-10(IL-10) and c ortisol in rats during gut ischemia/reperfusion. Methods Wistar rats were anaesthetized with soluble pentobarbitone, and subjected to superio r mesenteric artery occlusion (SMAO) for 60 minutes, followed by reperfusion f or 60 minutes. Animals were divided into three groups, pretreated group (carbach ol injection in
intestine at 30 minutes after SMAO; 0.1 mg/kg), treated gro up (
carbachol injection at 30 minutes from onset of reperfusion), and controls ( saline injection). Plasma TNF-α, IL-10 and cortis ol levels were determined at 1.0, 2.5 and 6.0 hours after SMAO. Results The plasma levels of TNF-α significantly decreased in pretreated and treated groups than those in controls after carbachol injection(both P <0.01). However, the levels of IL-10 and cortisol didn't show si gnificant differences among three groups. Conclusion The results suggest that carbachol can reduce the proinflammatory cytokine relea se and have a less inhibitory effect on the anti-inflammatory cytok ine. It is indicated that carbachol play a potential role in alleviating syste mic inflammatory response during splanchnic ischemia/reperfusion injury.