AIM:To observe the symptoms of
neurological impairment induced by cerebral hemorrhage and the changes of
mitochondrial membrane
potential so as to investigate the curative effect of Pinggan Xifeng Tang.METHODS:Cerebral hemorrhage models of rats were established by stereotaxical injection of collagenaseVII into the pallidum.
Neurological function was scored by neurological impairment scale.Fluorescent intensity of
mitochondrial suspension was determined with spectrofluorometer for testing the membrane potential.RESULTS:When the rats had cerebral ischemia, there quickly appeared the symptom of nerve damage.Pinggan Xifeng Tang functioned clearly to decrease the scores of neurological impairment(4 h:8.80 scores,1 d:6.80 scores,3 d:2.80 scores,7 d:1.60 scores).The membrane potential of mitochondria decreased greatly 4 hours after operation,decreased to the lowest level one day after operation,and restored gradually at 3-7 days after operation, but was still lower than that of the normal and pseudo-operation groups (t=7.352, P< 0.05).The mitochondrial membrane potential of the Pinggan Xifeng Tang group was obviously higher than that of the model group 1,3,and 7 days after ischemia , respectively(t=2.583,P< 0.05). CONCLUSION:The mitochondrial membrane potential of the cerebral ischemiac rats decreases;Pinggan Xifeng Tang can inhibit the decrease, and at the same time, relieve the nerve damage.