Objective: To investigate the protective effect of
angelica sinenisis injection on hematopoiesis of aplastic anemia(AA) mice.
Methods: The female Balb/c mice were divided into 5 groups. Group A was normal control. Group B had been generally irradiated by sub-lethal dosage 60 Co 6 Gy γ-ray, then injected through tail vein with mixed lymphocytes of thymus gland and lymphoid node of DBA mice in 4 hours. After AA models were established, each mouse was
intraperitoneally injected with sterile saline 10 ml·kg -1 ·d -1 for 12 days. Mice from Group C were intraperitoneally injected with
angelica sinenisis injection 2 ml·kg -1 ·d -1 for 12 days for an intervention treatment. Mice from Group D were intraperitoneally injected with angelica sinenisis injection 10 ml·kg -1 ·d -1 for 12 days. Mice from Group E were intraperitoneally injected with testosterone propionate 10 ml·kg -1 ·d -1 . All mice were killed by cutting neck on the 12th day, and ulnas and femur were taken out. The bone marrow ultrastructure of the mice ulnas were observed. The number of femnr bone marrow mononucleate cells(BMNC)were measured and the CD34 expression levels were measured by the flow cytometor analysis system. Results: The expression levels of CD34 in Group B were lower than those in Group A (P<0.01), but they were increased in Group D (P< 0.01 ). The ultrastructure observation showed that the mitochondria and endoplasmic reticulum of hematopoietic cells in Group B had been reduced and aberrant, and the blood sinus cavity was expanded and the wall of sinus was broken. In Group D, the mitochondria and endoplasmic reticulum were plentiful and normal in shape, and the blood sinus were almost normal. No obvious difference was found between Group D and Group E in MNC counting and hematopoietic microenvironment. Conclusion: Angelica sinenisis injection plays a protective role in aplasitic anemia mice .Because it could promote the proliferation of bone marrow of mice, it could improve the hematopoietic microenvironment.