Objective It is to study the effect and its mechanism of
green tea extract on glucose tolerance in
diabetic rats.Methods The effect of green tea extract on glucose tolerance of diabetic rats was observed after the diabetic rats were took orally with sucrose or starch and green tea extract. Then the
inhibitory activities of green tea extract on
alpha-glucosidase (EC 3.2.1.20) and
alpha-amylase (EC 3.2.1.1) were determined with colorimetric method.Results Green tea extracts significantly improved the blood sugar level of the diabetic rats loaded with sucrose or starch. In inhibitory test of
diabetes correlative enzyme, green tea extract showed stronger inhibitory activity on alpha-
Glucosidase and its IC 50 value was 0.13 g/L. While the inhibitory activity of green tea extract on alpha-amylase was faintish and its inhibitory rate was 21% at the IC 50 value of 1 g/L.Conclusion Green tea extract can reduce the blood sugar level of the diabetic rats loaded with glucose, the mechanism maybe the inhibitory effects on alpha-glucosidase and alpha-amylase.
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