AIM : To study mRNA expression of ATP-sensitive potassium channels (K ATP) of
pulmonary artery in
chronic hypoxic pulmonary artery hypertension (CHPAH) rats and in chronic hypoxic rats treated with novel K ATP opener
Iptkalim hydrochloride. METHODS : Sixteen Sprague-Dawley(SD) male rats were randomly divided into control group,
Hypoxic group, low dose Iptkalim group ( 0.75 mg·kg -1·d -1), and high dose Ipkalim group ( 1.5 mg·kg -1·d -1). Except the first group, the other three groups were put into hypoxic and normobaric chamber to establish
Chronic hypoxic model. Four weeks later, reverse transcription polymerase chain reaction (
RT-PCR) was performed to analyze the mRNA level of K ATP channels in pulmonary main artery smooth muscles. RESULTS :The mRNA levels of SUR2 in the hypoxic group were significantly lower than those in the control group, this decrease was completely reversed by Iptkalim in high dose group, and no difference in Kir 6.1 mRNA level was found between four groups. CONCLUSION : K ATP channel transcriptional expression is inhibited by chronic hypoxia, and this inhibition can be up-regulated by Iptkalim. The role of potassium channel in the development of CHPAH may explain the therapeutic mechanism of Iptkalim.
Published: April 26, 2004
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