AIM:To prepare the turbutaline sulphate pulsatile controlled-release pellets (TB-PCRP). METHOD:The turbutaline sulphate pulsatile controlled-release drug delivery system were prepared by multile- layer coated technology with L-HPC as the inner coating swelling layer and ethylcellulose aqueous dispersion as outer coating controlled layer. The process was studied by orthogonal experiment design using orthogonal form L 9(3 4)with the lag time and the drug accumulated release as the evaluation quota to decide the best preparing condition,and the concentration of sodium dodecyl sulfate(SDS),the coating level of the swelling layer and the controlled layer as effecting factors. RESULT: The drug release is triggered while rupturing the membrane of controlled layer. The lag time and the accumulated release of turbutaline sulphate from the TB-PCRP are influenced obviously by the concentration of SDS,the coating level of the inner swelling layer and the outer controlled layer. Three batches of the optimum formulation were produced,and the release behavior determined \%in vitro\% of the TB-PCRP could meet all the requirements with a lag time of 4.5 h and more than 80% drug accumulated release within following 1.5 h under the simulated gastrointestinal pH conditions by using L-HPC and 0.05 mol/L SDS as the inner swelling layer with the 16%(w/w) coating level and the ethylcellulose aqueous dispersion as the outer controlled layer with the 18%(w/w) coating level. CONCLUSION: The results showed that the TB-PCRP prepared could meet all the requirements,and the methods of the optimization are useful for the turbutaline sulphate pulsatile controlled-release drug delivery system.