Objective: To explore gradient extraction of grape seed proanthocyanidin and their antioxdative effect. Method: After sample grape seeds were extracted with ethanol, the active components were obtained by application of different organic solvents. Through investigation of the free-radical scavenging effect of ethylacetate components, the optimum concentration range was determined by DPPH method and a comparison was made on the antioxidative effects of the different components from grape seeds. Result: The optimum assay range was 1.0～10 ug/ml with the scavenging rate between 25%～80%. The sensitivity and accuracy of this method were satisfying. The free-radical scavenging effects of different components from grape seed proanthocyanidin came in the following : Ethyl acetate> Acetone> Ethanol> Ether> the primary extracts of ethanol > Dichloromethane> Petroleum ether. Among all components, the activities of Ethyl acetate and Acetone extracts were excellent marked by the active components highly concentrated. Conclusion: Gradient extraction of grape seed proanthocyanidin was very promising.