In order to probe further into the role of PMBE inhibiting the growth of cancer,and construct the pharmacological animal
model,combination of cell culture,multiple group MTT assays and computer modeling techniques was used in this present study to assess the effect of PMBE,FBS and t on the cell
Growth Inhibition Ratio(Gir) of human colorectal carcinoma LoVo cells cultured in vitro.Quadratic general rotational design at 5 levels of 3 factors was used to design the MTT assay scheme.The reaction model between the GIR(Y) and PMBE(X_1),FBS(X_2) and t(X_3) was established.Optimal combination of the three factors,effects of single factor and interaction between them were explored respectively,and appropriate combination of different factors could suppress the cell growth effectively.By simulation with computer,the optimal combination of PMBE,FBS and t with the highest GIR 0.42 was found at 0,1,0 level,or 140?μg/mL,15% and 48?h,respectively.A law of "reward descending of inhibitor effect" and nonlinear characteristic of many factors' integrative effect during cell treatment were first put forward.This research is a novel attempt to apply statistics principle in design,measurement and evaluation(D.M.E.) fields of medical scientific research,with reference significance in determining the dosage of candidate drug for pharmacological animal model.