Objective:To observe the abirritation and anti-inflammation effects of capsaicin and its analogues.Methods:The effect of capsaicin,dissolved in alkali water,on writhing response of mouse;The effect of capsaicin solution on pain threshold of mouse and rat were tested by hot-plate test and tail flick test separately;The effect of capsaicin ointment on the pain threshold of rat was tested by using formalin pain model.Using and acute inflammatory model,in which the mouse ear edema was induced by dimethylbenzene,tested the anti-inflammation effect of capsaicin solution.Using an acute inflammatory model,in which the rat feet swelling induced by formalin,tested the anti-inflammation effect of(capsaicin) ointment on rat feet swelling.Result:The capsaicin solution(0.01%,0.03%,0.05%)can restrict the writhing response(P<0.01)and prolong the pain threshold of mouse and rat remarkably(0.03%-P<(0.05),0.06%-P<(0.01),0.09%-P<0.001);the capsaicin ointment can prolong the pain threshold of rat remarkably(0.01%-P<(0.05%,)0.03%-P<0.001,0.05%-P<0.0001).The capsaicin solution(0.05%)can restrict ear edema of mouse(obviously)(P<0.05)and capsaicin ointment(0.09%)can restrict feet swelling of rat obviously(P<0.05).Conclusion:Capsaicin and its analogues have distinct abirritation and anti-inflammation effect on moust and rat.