Purpose An
HPLC/MS/MS method was developed to measure the concentration of
Hupernize A', 1, 1594179)"; href="/tags/hupernize-a/">hupernize A in mouse plasma and brain.The method was used to preliminarily study
pharmacokinetics of hupernize A in mouse plasma and brain. Methods Samples were extracted liqued-liqued extraction and separated on PHENOMENEX C_(18) column.The mobile phase is methanol∶water=60∶40.Quantitation was based on multiple reaction monitoring(MRM) using the precursor→production combination of m/z 2 43→ 154 and m/z 278→ 203 for Hupernize A and Clenbuterol respectively. Results 1.The method has a linear range from 1~1 000 ng·mL~(-1)(r=0.999 0) and minimal detectable concentration at 1 ng/ml.The intra-day and inter-day RSD were less than 7%.The method can be applied to determine plasma
concentrations and brain concentrations.2.The plasma concentrations vs time curve was fitted as an open one-compartment model.The pharmacokinetic parameters of mouse plasma were 6min for T_(max),281 ng·mL~(-1) for C_(max),35 266 ng·L~(-1)·min~(-1) for AUC_((0→tn)),AUC_((0→∞))=38 056 ng·L~(-1)·min~(-1) and t_(1/2)=99 min;The brain concentrations vs time curve was fitted as an noncompartment model.The pharmacokinetic parameters of mouse brain were 60min for T_(max),224 ng·mL~(-1) for C_(max),(44 344) ng·L~(-1)·min~(-1) for AUC_((0→tn)) and AUC_((0→∞))=58 571 ng·L~(-1)·min~(-1). Conclusions The method is sensitive,simple and rapid.It can be applied to study the pharmacokinetics of hupernize A.Hupernize A was eliminated more slowly in mouse brain than in mouse plasma.
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