Effect of Component F_(022) in Radix Isatidis on the Expression Upregulation of Moesin mRNA in Mice Article Abstract
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Published: January 25, 2006
Objective To probe into the effect of the anti-endotoxic part in Radix Isatidis on the expression of moesin mRNA in tissues of mice induced by lipopolysaccharide(LPS). Methods One week before the test,an intraperitoneal injection of 0.2 mL·(20 g)~(-1) bacillus calmettc-guerin(BCG) was given to each of 30 mice,which were then randomized equally into 5 groups.Mice of the test groups were given 0.4 mL·(20 g)~(-1) of 1.00%,0.50% and 0.25% solution of F_(022) by gastrogavage,mice of the control group were given 0.4 mL·(20 g)~(-1) of 1.00% solution of F_(022) by gastrogavage,and mice of the model group were given each an equal volume of 0.9% sodium chloride.The above procedures were repeated 1.5 h later,and 30 min following the second drug administration,the test groups and the model group were given 0.2 mL·(20 g)~(-1) of LPS.Mice were anesthetized 9 h later by ether with the liver,kidney and spleen tissues treated by moesin mRNA hybridization in Situ and the staining of cytoplasm observed under microscope. Results A brown yellow staining of the tissue cytoplasm was taken as positive,and the administration of LPS could increase moesin mRNA expression.With prior administration of F_(022) part in radix isatidis the moesin mRNA expression by LPS was inhibited with a dose dependent characteristic. Conclusion The F_(022) part in radix isatidis can inhibit the LPS induced moesin mRNA expression in tissues of mice liver,kidney and spleen with an anti-endotoxic mechanism possibly at the molecular level.