Objective To investigate the effect of astragaloside,one of the active components of astragalus membranaceus,on
myocardial nerve growth factor(NGF)expression in murine myocarditis model with coxsackievirus B(CVB_3).Methods One hundred Balb/c mice were randomly divided into 6 groups.Group A
were served as normal controls group and group B(n=10,treated with 9% astragaloside 0.1 mL,ig,for 1 week)as high-dose control.Eighty infected mice treated with CMC and 1%,3%,9% astragaloside 0.1 mL,ig,for 1 week (n=20 in each group)were served as group C(myocarditic control group),D(low-dose intervention group),E(middle-dose intervention group),F(high-dose intervention group),respectively.The mice were killed and their hearts were removed after 14 days.The expression levels of NGF mRNA and protein in the myocardium were examined by RT-PCR and immunohistochemistry,respectively.Results The mortality was significantly reduced in 9% astragaloside treated infected mice that was 10%(2/20)vs 45%(9/20)in group C(P<0.05).In uninfected mice,9% astragaloside did not cause any death.The expression levels of NGF mRNA,protein and myocardial histopathologic scores were significantly lower in group F than those in group C.However,the NGF expression and myocardial histopathologic scores were not affected by 1%(and 3%) astragaloside.Conclusion Astragaloside might play a pivotal role in protecting the heart injure in Balb/c mice myocarditis with CVB_3 by suppressing excessive expression of NGF.
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