AIM: To investigate the effects of D-glucose on monocyte chemoattractant protein-1(MCP-1),intercellular cell adhesive molecule
type-1(ICAM-1),vascular cell adhesive molecule1-1(VCAM-1)mRNA expression and the protective effects of arecoline,PPVP,DMHPPP and simvastatin in cultured bovine aorta
endothelial cells,thereby to explore possible mechanisms by which the compounds prevent the formation of diabetic vascular complications.METHODS: Bovine aorta
Endothelial cells were cultured in vitro with indicated concentration of D-glucose for indicated time.After the cells were harvested at the specified situation,the MCP-1,ICAM-1 and VCAM-1 mRNA expression was detected by reverse transcription-polymerase chain reaction(RT-PCR) and normalized to the mRNA level of β-microglobulin in the absence or in the presence of D-glucose or land arecoline,PPVP,DMHPPP and simvastatin,respectively.RESULTS: The expression of MCP-1,ICAM-1 and VCAM-1 mRNA all increased dose-and time-dependently after treatments with 10,25,50(mmol·L~(-1)) D-glucose for 16 h and 25(mmol·L~(-1)) D-glucose for 8,16,24 h,respectively.Compare with control group,after the bovine aorta endothelial cells were incubated with 10-5(mmol·L~(-1)) arecoline,PPVP,DMHPPP,simvastatin for 20h in advance,respectively,the injury effects which were subsequently induced by 25(mmol·L~(-1)) D-glucose for 16h could be prevented.CONCLUSION: Cultured bovine aorta endothelial cells can express MCP-1,ICAM-1,and VCAM-1 mRNA at a low level,and high concentration of D-glucose can induce excess expression.The regulation effects of arecoline,PPVP,DMHPPP and simvastatin on the excess expressions are different,respectively.