Objective To study the anticancer activity of Aidi(AD) injection and probe into its immunoenhancing effect as well as the corresponding mechanisms. Methods Experiment①: 50 Balb/c mice were randomly divided into 5 equal groups: control group,model group and three trial groups.Mice of the model group and trial groups were given each a hypodermic injection of 0.1 mL of H_(22) tumor cell suspension(containing 2×10~(7) cells·mL~(-1))at the subaxillary region.Mice of the control group were given no injection.24 hours later,mice of the three trial groups were given each an intraperitoneal injection of AD injection at lose dose(1 g·kg~(-1) in 0.2 mL),medium dose(2 g·kg~(-1) in 0.2 mL) and high dose(3 g·kg~(-1) in 0.2 mL),respectively.The injections were given once daily for 5 consecutive days.Mice of the control group and model group were given intraperitoneal injections of equivalent amounts of 0.9% sodium chloride solution q.d.for 5 days as well.On the 5~(th) day of the experiment,mice of all the 5 groups were given each an intraperitoneal injection of 0.2 mL of 20% SRBC(sheep red blood cells) suspension and the afore mentioned treatment for different groups of mice was continued for another 5 days.On the 10~(th) day,blood samples were taken from the postorbital venous plexuses of the animals.The value of absorbance at the 50% hemolytic dose(HC_(50)) was assayed with the antihemolysin antibody method.Experiment②: 50 Balb/c mice were divided and inoculated with suspensions of H_(22) tumor cells in the same manner as described in Experiment ①.
24 h later,mice of the 3 trial groups were given intraperitoneal injections of AD injection at the same low dose,medium dose and high dose,respectively,as described in Experiment ①.These injections,however,lasted 11 days.Mice of the control group and model group were given intraperitoneal injections of equivalent amounts of 0.9% sodium chloride solution for 11 days as well.On the 12~(th) day of the experiment,the body weight of each mouse was noted down and the animals were sacrificed after blood samples had been taken.The spleen index and thymus index were calculated and serum concentrations of IgG,IL-2,TNF-α determined with ELISA. Results The HC_(50) value of the mice of the control group and three trial groups treated with AD injection were significantly higher than those of the mice of the model group(P<0.01).The spleen index and thymus index of the mice of the control group and three trial groups were also strikingly greater than those of the mice of the model group(P<0.05 or P<0.01).Besides AD injection was shown to dramatically increase the production of IgG,IL-2 and TNF-α in tumor-bearing Balb/c mice. Conclusion AD injection was shown to strikingly enhance the immune function of tumor-bearing mice.