Objective To assess the feasibility of using anti-TGF-β1 polyclonal
antibody as an assistant therapy after
laser canalicular plastic operation. Methods KTP/YAG laser canalicular plastic surgery was performed at fixed power with combination of injection of anti-TGF-β1 polyclonal antibody or mitomycin-C(MMC) to rabit's lacrimal canaliculus.And those operated without any injection were taken as the control group.The histopathalogy of lacrimal
canaliculus was studied by means of HE staining.Immunohistochemical technique was used to detect the
distribution and expression of TGF-β1 and TGF-β2.Picrosirius method was used to detect the
collagen content in canalicular tissue.The half-quantitative analysis of the distribution and expression of TGF-β1、TGF-β2 and collagen were assessed by HMIAS-2000 color pathologic analysis system. Results The expression of TGF-β1 and TGF-β2 was significantly suppressed on day 1 to week 1 after operation in the group of anti-TGF-β1 antibody or mitomycin-C.The content of collagen in group of anti-TGF-β1 antibody or mitomycin-C was lower than that of control group after week 1. Conclusion Mitomycin-C and anti-TGF-β1 antibody can influence the distribution and expression of TGF-β1 and TGF-β2,and in a certain degree,inhibit the hyperplasia of collagen fibers,thus reducing the blocking of canaliculus.Therefore,anti-TGF-β1 antibody has a potential as a new assistant therapy for reducing post-surgical scarring.
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