Shvoong Home > Medicine & Health > The mechanism and treatment phases chosen of glycine for inhibition lipopolysacharide induced Kupffe Summary

.

The mechanism and treatment phases chosen of glycine for inhibition lipopolysacharide induced Kupffe Article Abstract

Abstract by : TsingHua
Visits : 14  words: 300   Published: February 01, 2006
Objective To explore the possible mechanism and optimal treatment phase of glycine for inhibition lipopolysaccharide (LPS) induced Kupffer cells (KCs) activation. Methods The KCs were isolated from 40 BALB/c mice and divided into four groups: the endotoxin group, the prevention group, the early treatment group and the later treatment group (n=10). The endotoxin group was treated with 10 mg/L LPS, and in the other three groups, glycine (1 mmol/L) was given 24 h before, or at 0 h or 4 h respectively after LPS stimulation. At 0 h, 1 h, 2 h, 6 h and 12 h after LPS stimulation, the mRNA levels and protein expression of interleukin-1 receptor associated kinase-4 (IRAK-4) were determined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot respectively, and nuclear factor-kappaB (NF-κB) activities as well as tumor necrosis factor alpha (TNF-α) levels were also detected by enzyme-linked immunosorbent assay (ELISA). Results The climax values of IRAK-4, NF-κB and TNF-α were significantly higher in the endotoxin group and the later treatment group than that in the other two groups (t=3.17, 4.33, 2.47, 126.73, P<0.01). Conclusion The results indicated that prophylactic or simultaneous treatment with glycine could effectively inhibit LPS-induced KCs activation by inhibiting IRAK-4 expression.

More abstracts about the The mechanism and treatment phases chosen of glycine for inhibition lipopolysacharide induced Kupffe
Please Rate this abstract : 1 2 3 4 5


Add your comment No comments

Comments & Reviews about The mechanism and treatment phases chosen of glycine for inhibition lipopolysacharide induced Kupffe Article Abstract

Read Free Summaries - Write and Get Paid

Summarize Human Knowledge on Shvoong. Join us!

------