Objective: To observe the effects of
tetramethylpyrazine on the content of tumor necrosis factorα(TNFα) and the activity
of myeloperoxidase(MPO) after
cerebral ischemia/reperfusion in rats,and to investigate its mechanism of protective effects on cerebral ischemia/reperfusion.Methods: Thirtysix male SD rats were randomly divided into shamoperated group,model group and(
tetramethylpyrazine) group.The thread with embolus was(inserted) in the cerebral(middle) artery in shamoperated group,but the artery was not(embolized).The cerebral ischemia/reperfusion models of rats were established in model group and(tetramethylpyrazine) group,while in the latter group,33 mg/kg of tetramethylpyrazine was(injected)(intraperitoneally) 30 minutes before ischemia.The methods of radioimmunoassay and pectrophotometry were used respectively to detected the content of TNFα and the activity of MPO in brain tissues of rats.(Results): Compared with those in shamoperated group,the content of TNFα((0.601±0.089) μg/L vs.(1.576±0.153) μg/L) and the activity of MPO((0.026±0.008) U/g vs.(0.409±0.044) U/g) in brain tissues(increased) markedly in model group(both P<0.01).Compared with those in model group,the content of(TNFα)((1.035±0.092) μg/L) and the activity of MPO((0.293±0.039) U/g) in brain tissues decreased(significantly) in the tetramethylpyrazine group(both P<0.01).Conclusion: Tetramethylpyrazine can(alleviate)(inflammatory)(reaction) and has neuroprotective effects on cerebral ischemia/reperfusion injury in rats,the(mechanisms) being possibly by decreasing the content of TNFα and the activity of MPO in the(brain)(after)(injury).