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Shvoong Home>Medicine & Health>In vitro effects of propofol on apoptosis and Bax expression induced by TNF-α in mouse spinal cord n Review

In vitro effects of propofol on apoptosis and Bax expression induced by TNF-α in mouse spinal cord n

Article Review   by:TsingHua     Original Author: Academic Journal of Second Military Medical University
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Objective:To study the in vitro effects of propofol on apoptosis and Bax expression induced by TNF-α in mouse spinal cord neurons.Methods: Spinal cord neurons were isolated from fetal mice and cultured in neurobasal medium with B27 supplement.On the 7th day of culture,neurons were randomly divided into 6 groups:control group,propofol(50 μmol/L) group,TNF-α group,propofol(25 μmol/L) with TNF-α group,propofol(50 μmol/L) with TNF-α group and propofol(100 μmol/L) with TNF-α group.Propofol with different concentrations was incubated with cultured cells for 30 min,then TNF-α was added with the final concentration of 2 000 U/ml for another 24 h incubation.Apoptosis was detected by PI/Hoechst33342 double staining technique and fluorescence microscopy.Bax expression was determined by immunocytochemical technique.Results: The apoptosis rate and expression of Bax in TNF-α group were both increased compared with those in control group((<21.8±1.1>%) vs <2.8±0.8>%,P<0.01;<0.251±0.016> vs <0.141±0.015>,P<0.01).The apoptosis rates and expression of Bax in 3 propofol groups(25,50,100 μmol/L) were(<16.2±1.2>%,<15.3±0.6>% and <12.2±0.8>%) and ((<0.198±0.011>,)<0.188±0.012> and <0.167±0.014>),respectively,and they were all significantly decreased compared with those in TNF-α group(<21.8±1.1>% and <0.251±0.0163>,all P<0.05).Moreover,a dose-dependent manner was observed in the above changes.Conclusion: Propofol within clinical concentration ranges may inhibit the apoptosis of spinal cord neurons induced by TNF-α through modulating the expression of Bax.
Published: February 28, 2006   
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