Objective To study the blood concentration and tissue distribution of total alkaloids of Lianzixin(TAL) after intragastric and intravenous administration.Methods The plasma and tissue concentrations of TAL were detected by using reversed phase high performance liquid chromatography method and the data was dealt with drug and statistics program to calculate the pharmacokinetics parameters and select apartment model.Results The time-concentration curve of TAL(20 mg/kg)~()intravenous administration corresponded to the model of three apartments.The main pharmacokinetics parameters were: t_(1/2α)=0.367 min,t_(1/2γ)=841.597 min,CL=2.336 L/min,AUC_(0-1080)=639.392(mg·min)/L,AUC_(0-∞)=823.754(mg·min)/L and MRT_(0-∞)=866.354 min.After intragastric administration of TAL (20 mg/kg),the parameters were as follows: AUC_(0-1080)=508.578(mg·min)/L,AUC_(0-∞)=787.035(mg·min)/L and MRT_(0-∞)=1466.040 min.The calculated absolute bioavailability was 80 %.TAL,after intragastric administration at 20 mg/kg,was quickly distributed to main organs,highest in liver at 10 or 30 min after administration,followed by lung and heart,lowest in brain at 30 min or 1 h.Meanwhile,the concentrations in other tissues were increased gently.After intravenous administration of TAL,the highest level appeared in the lung,followed by heart and kidney.At 5 min,a concentration peak occurred in heart,lung and kidney tissues separately,and dropped subsequently,then re-ascended to a higher level at 4 h.Conclusion The pharmacokinetics characters of TAL are extensive distribution,quick transfer,long in t_(1/2α)and large in Vc.The concentration of TAL in all tissues,except brain,is higher than that in plasma.