AIM: To observe the effect of liliaceous herb ethanol extraction, fragrant solomonseal rhizome extract A (EA-PAOA), on the 72
hour survival rate, serum tumor necrosis factor alpha (TNF-α) and nitrogen monoxide (NO)
levels of
mice with endotoxemia.METHODS: The experiment was completed in the Laboratory of Immunology, Jinzhou Medical College from January to March 2005. ① Totally 150 male kunming mice aged 6-8 weeks were divided into
group Ⅰ with 100 mice and group Ⅱ with 50 mice. ② Mice in group Ⅰ were divided into 4 groups:
model group and EA-PAOA (provided by Pro. Pan Xing-yu from Jinzhou Medical College)
groups of low, moderate and high dosage with 25 in each group. Mice in EA-PAOA groups were intravenously injected with 0.5, 1 and 2 g/kg EA-PAOA once a day for 7 successive days respectively, and mice in model group were intravenously injected with the equal volume of saline. One hour after the last medication, mice in model group and EA-PAOA group were intravenously injected with 10 mg/kg endotoxin to establish endotoxemia model so as to record 72-hour survival rate of mice. ③ Mice in group Ⅱ were divided randomly into 5 groups: normal control group, model group and EA-PAOA groups of low, moderate and high dosages with 10 in each group. Administration in model group and EA-PAOA groups were the same as that in group I, and mice in normal control group were treated with the equal volume of saline. Levels of TNF-α in serum were quantified by sandwich enzyme-linked immunoadsordent assay (ELISA) method, and NO level was detected by nitrate reductase chemical colorimetry 6 hours after injection of endotoxin. ④ One way analysis of variance was employed in significance test of different groups, followed by LSD analysis with equal variances and Dunnet T3 test with unequal variances.RESULTS: All 150 kunming mice entered the final analysis. ① 72-hour survival rates in EA-PAOA groups were higher than those in model group (P < 0.05), and no significant difference at three EA-PAOA dose groups was shown (P > 0.05). ② In model group, levels of TNF-α and NO were higher than those in normal control group (P < 0.01), but those in EA-PAOA dosage groups were lower than those in model group which showed dosage dependence (P < 0.01). NO levels in moderate and high EA-PAOA groups were lower than those in model group (P < 0.01), but difference of NO level in low EA-PAOA group was not significant (P > 0.05).CONCLUSION: EA-PAOA can increase 72-hour survival rate of mice with endotoxemia, and decrease levels of TNF-α and NO, which shows dose dependence.
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