AIM: To observe the effect of Jiedu Shaoshang ointment, which has certain curative effect at superficial Ⅱ degree and deep Ⅱ degree, on transforming growth factor beta (TGF-β), micro-vascularization and collagen synthesis, and explore its possible effect pathway. METHODS: The experiment was conducted in Animal Center of Sun Yat-sen University and surgical Laboratory of the First Affiliated Hospital of Sun Yat-sen University from September to December in 2004. Ten little pigs were used to make deep second-degree burn models and all these wounds were randomly divided into Jiedu Shaoshang ointment observational group and blank control group. Wound healing time was recorded; wound bed tissues were got on the 3rd, 5th, 7th and 9th days respectively after burns. Expression of TGF-β was detected using inmmunohistochemistry streptavidin biotin-peroxidase labeled method. Five sights were selected under 400 power light microscope, and 100 cells were observed in every sight. It was positive staining in plasma or (and) nucleus. Quantity of positive cells was counted. Vascular endothelial cells were labeled with CD34-anti-immunohistochemistry. Filleting was observed fully under low power microscope (10×10) so as to define highest density of tissue vessel, and then counting was done under high power microscope (10×40)and the number of microvessel was recoded at the 5 sights. Average was gained; Density of microvessel was counted. Content of tissue collagen was detected with hydroxyproline method. Wound healing time, positive expressive rate of TGF β , counting of microvessel density at wound and content of collagen were compared between experimental group and control group.
RESULTS: Ten included animals were all involved in the result analysis, no drop-out animal. ①Healing time in the observational group was earlier significantly than that in the control group (11.0±1.5),(14.0±2.5)days, t =4.08,P < 0.001. ②The expression of TGF β was at inflammatory cell and epithelial cells of hair follicle. At the 3rd and 5th days after injury, the positive expressive rate of TGF β was higher significantly than that in the control group (25.0±7.5)%,(11.0±4.6)%;(45.7±8.3)%,(24.6±7.4)%,t=3.61,6.34,P < 0.01. The peak of expression of TGF β appeared earlier and stronger in the observational group than that in the control group. ③The counting of microvessel density at the 5th day was higher markedly in the observational group than that in the control group (40.6±2.4),(32.6±2.4) piece,t=2.69,P < 0.05. ④The content of collagen at the 7th and 9th days was higher remarkably than that in the control group (2.45±0.72),(1.97±0.15) g/mg;(3.51±0.46),(2.85±0.13) g/mg;t=3.61,6.79,P < 0.01. CONCLUSION: The Jiedu Shaoshang ointment can promote wound healing, which may be related to the elevation of expression of TGF-β, acceleration of hyperplasia of vessel and the synthesis of collagen in later stage.