AIM: To observe whether
sodium salicylate could induce the
apoptosis of adrenal gland chromaffin tumor cell (PC12), and primarily explore the possible mechanism. METHODS: The experiment was conducted in Wuhan University from July 2004 to November 2005. Rat adrenal gland chromaffin tumor cell (PC12) was observed. PC12 cell was cultured in the culture plate and was randomized after synchronization without serum. Then it was treated by sodium salicylate of various concentrations low
concentration (0.312 5, 0.625 mmol/L), high concentration (1.25, 2.5, 5 mmol/L) for 72 hours. The cell viability was determined by Methyl thiazolyl tetrazolium (MTT) assay, cell form was observed under inverted microscope and fluorescence microscope,
ratio of apoptosis was detected with flow cytometry, and the content of NF-κB was assessed by Western blotting. RESULTS: Treatment of PC12 cells with sodium salicylate for 72h, in the range of 1.25-5 mmol/L, caused a great decrease in cell survival (P < 0.001) and induced typical morphological changes of apoptosis. It was showed that the volume of the cells deflated and became round obviously, some cells changed from adherence condition to suspension condition while the character of photonasty weakened. The ratio of apoptosis detected with flow cytometry showed that the ratio of
apoptotic cells was decreased with the concentrations of sodium salicylate increasing. Compared with the control group, the ratio of apoptotic cells which had been treated with low concentration of the sodium salicylate (0.312 5, 0.625 mmol/L) did not increase obviously. But typical apoptotic peak appeared while disposal with high concentration of the sodium salicylate (1.25, 2.5, 5 mmol/L). The ratio of apoptotic cells was significantly increased as compared with the control group (P < 0.01). Moreover, it was concentration-dependent. The content of NF-κB in PC12 cells was decreased with the concentrations of sodium salicylate increasing.CONCLUSION: Sodium salicylate of high concentration can induce the apoptosis of PC12 cells in vitro. Sodium salicylate can decrease the NF-κB content in a concentration-dependent manner.
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