AIM: To investigate the effects of crescent euphorbia on the growth, immuno-regulation and blood system of tumor of mice with Lewis lung cancer, and the effect on cycle of tumor. METHODS: ① This experiment was completed in the Laboratory of Food and Drug Research Institute, Ocean University of China (Key Laboratory of Shandong Province) from November 2004 to January 2005. Seventy C57BL/6J pure mice were randomly divided into seven groups: blank control group, model group, 7.5, 15, 30, 60 g/kg crescent euphorbia extract groups and 1 mg/kg cisplatin group with 10 in each group. ② Mice in each group were injected subcutaneously into right axilla with 0.2 mL Lewis lung carcinoma suspension cultured for 14 days to establish models of gastric tumor. ③ From the next day of vaccination, mice in 7.5, 15, 30, 60 g/kg water extract of crescent euphorbia were perfused with 7.5, 15, 30, 60 g/kg water extract of crescent euphorbia respectively for 12 days, and mice in 1 mg/kg cisplatin group were injected intravenously with 1 mg/kg cisplatin once every day for 7 days. Total crescent euphorbia was provided by Qingdao Tongrentang Dispensary (place of production: Jiangsu batch number 040901), and by Beijing Jingsheng Traditional Chinese Medicine Pharmacy Factory (certification: ji Y20040006) water extract of crescent euphorbia: 500 g raw crescent euphorbia was added with water, boiled, filtered, and concentrated, then oral liquid was obtained. 1.0 g raw crescent euphorbia was contained in 1 mL oral liquid. Diet and growth of tumor of mice were observed every day. ④ 24 hours after last perfusion, mice were weighted and killed. Tumor was weighted and anti-tumor rate was calculated as follow: (average weight of tumor in model group-weight of tumor in treatment group)/ average weight of tumor in model group × 100%. Mice were sacrificed after 12-day perfusion. Thymus and spleen of mice were taken out and weighted to calculate index of spleen index of spleen = weight of spleen/ body mass (mg/g) and index of thymus index of thymus = weight of thymus/ body mass(mg/g) (body mass was the difference between body mass of mice and tumor mass). ⑤ After 12-day perfusion, blood cell from eyeball blood was analyzed with blood cell analyzer.
⑥ Cell cycle and apoptosis rate of tumor tissue were assayed with FACSort flow sytometer. ⑦ Measurement data in groups were compared with analysis of variance, and data among groups were compared with paired t test. RESULTS: Totally 70 mice entered the final analysis. ① The results showed that tumor masses in 30 and 60 g/kg water extract of crescent euphorbia groups and 1 mg/kg cisplatin group were lighter than that in model group (P < 0.05-0.01). Anti-tumor rates in 7.5, 15, 30, 60 g/kg water extract of crescent euphorbia groups were 0.608%, 16.931%, 32.806% and 58.255% respectively, and the rate was increasd along dosage increasing. ② Water extract of crescent euphorbia in each dosage groups had no effect on index of thymus, and indexes of spleen of mice in 30 and 60 g/kg water extract of crescent euphorbia groups were lower than that in model group (P < 0.05). ③ There were not significant differences on numbers of leucocyte, erythrocyte, platelet and ferrohemoglobin in each dosage group as compared with those in model group (P > 0.05), but number of leucocyte in 1 mg/kg cisplatin group was less than that in model group (P < 0.01). ④ Percentages of cells during DNA synthesis in 30 and 60 g/kg crescent euphorbia groups and cisplatin group were higher than that in model group but was lower than that in model from quiescent period to DNA presynthetic phase. Apoptosis rates in 30 and 60 g/kg crescent euphorbia groups and cisplatin group were higher than that in model group (P < 0.05). CONCLUSION: Water extract of crescent euphorbia can inhibit the growth of Lewis lung cancer in mice, and the effect is strengthened along dosage increasing. It also has immunoloregulation but does not have side effects on the blood syste