AIM: To observe the effect of the litchi nucleus
extract fluid on blood glucose, blood lipid, cerebral protein, brain homogenate,
glutathion peroxidase (GSH-Px) and oxygen free radical (O2-) of diabetic mice induced by alloxan.METHODS: ① The experiment was finished in the Laboratory of Biochemistry, Youjiang Medical College for Nationality from April to May 2005. The laboratory was the key laboratory of Guangxi Zhuang Autonomous Region. Totally 40 mice were selected and were divided into 2 groups: 10 as control group and 30 as model group. Animals in model group were given with 200 mg/kg alloxan after lysis of distilled water. When fasting blood glucose reach to 8.10 mmol/L 3 days after injection, the modeling was successful. Then 24 mice were divided randomly into 3 groups high-dosage, low-dosage
treatment group and model group with 8 in each group. In high-dosage group, mice were perfused with 0.3 mL litchi nucleus
extract fluid, mice in low-dosage group were perfused with 0.1 mL litchi nucleus extract fluid and 0.2 mL distilled water, once a day for 4 successive days. Mice in model and normal control groups were perfused with 0.3 mL distilled water, once a day for 4 successive days. ② Fasting blood glucose was assayed with toluamide method before and 3 days after modeling and 2 and 4 days after treatment respectively. After 4-day treatment, triglyceride (TG) was assayed with GPO-POD method total cholesterol (TC) with COD-CE-PAP method high-density lipoprotein cholesterol (HDL-C) with precipitation titration protein of brain with biuret method the activity of gluathion peroxidase in brain homogenate with the speed of enzymatic reaction content of oxygen free radical with α-naphthylamine. ⑤ Measurement data were compared with analysis of variance.RESULTS: After injection alloxan, 2 mice died, 4 mice failed to model, and totally 34 mice entered the final analysis. ① Fasting blood glucose: There was no significant difference before modeling in each group (P > 0.05) but that in low-dosage and high-dosage groups was lower than that in model group after 4-day treatment, and the value was close to that in normal control group (P > 0.05). ② Blood lipid after 4-day treatment: TC level was not significant difference in each group (P > 0.05) but HDL level in high-dosage groups was higher than that in model group and low-dosage group (P < 0.05,0.01). ③ Biochemical indexes of brain homogenate after 4-day treatment: Level of GSH-PX in normal control group, high-dosage and low-dosage groups was higher that that in model group (P < 0.05), but content oxygen free radical was lower than that in model group (P < 0.05).CONCLUSION: ① The litchi nucleus extract fluid can decrease level of blood glucose in diabetic mice induced by alloxan and has the function of regulating the disorder condition of blood lipid. ② The litchi nucleus extract fluid has a certain effect on clearing oxygen free radical and improve anti-oxidation ability.