AIM: To compare the anti-fatigue effects of extractions from two kinds of bivalves on
mice by observing the swimming time of mice with
weight loading, level of blood urea nitrogen (BUN) and the content of hepatic glycogen in mice fed with emerald mussel extractions (EME) and scallop extractions (SE). METHODS: The experiment was performed in the Experimental Animal Center and Institute of Biochemistry and Molecular Biology of Guangdong Medical College from February to June 2005. ①240 male mice of Kunming SPF grade were selected. Experimental scallop and emerald mussel were bought from markets. Two kinds of buff powder were obtained by extracting, column chromatography, condensation and drying. Two extractions were proved to be safe by acute toxicity testing.②Test of swimming with weight
loading in mice: Eighty included mice were divided randomly into 2 groups: SE
group and
eme group, with 40 mice in each group Each treatment group was randomly subdivided into 4 subgroups: 10, 20 and 40 g/L SE and EME group (respectively fed with 10, 20 and 40 g/L SE and EME water-solution freely), mice in the
control group were fed with boiled water freely. After 30-day feeding, mice were put in pools to swim at (25±1) ℃ with their tails loaded with 5%-body-mass plumbum wire. Time from swimming to sinking into water were recorded and taken as weight loading time. ③Determination of BUN in mice:
groups of mice were the same as before, level of BUN in mice was measured with diacetylmonoxime coloration after cutting the heads.④Test of hepatic glycogen in mice: Groups of mice were the same as before, hepatic glycogen in mice were assayed with oxidase after cutting the heads. ⑤Differences of measurement data were compared with t test and t' test while in heterogeneity of variance. RESULTS: 240 mice were involved in the analysis of results.①Swimming time of mice with weight loading in 20 and 40g/L SE groups were longer than that in the control group(P < 0.05, 0.01). Swimming time of mice with weight loading in 20 and 40 g/L EME groups were longer than that in the control group (P < 0.05, 0.01). Time in the 40 g/L SE group was longer than that in the EME group (P < 0.05). ② Level of BUN in mice of 40 g/L SE group and 20 g/L EME group was obviously lower than those in the control group (P < 0.05). Levels of BUN in mice of 40 g/L SE group was significantly lower than that in the 40 g/L EME group (P < 0.05). ③Contents of hepatic glycogen in mice of 20, 40 g/L SE and EME groups were markedly higher that those in the control group (P < 0.05). Differences in contents of hepatic glycogen in mice of SE and EME groups of same concentration were not significant (P > 0.05).CONCLUSION: Both SE and EME have anti-fatigue effects on mice, while the anti-fatigue effect of SE is better than that of EME.
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