Impact of extract of ginkgo biloba leaves preconditioning on the expression of TNF-αmRNAand FasmRNA Article Abstract
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Published: February 28, 2006
To investigate the protective effects and the impact on the expression of TNF-αmRNA and FasmRNA by extract of Ginkgo Biloba leaves preconditioning in rat liver transplantation. Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation(OLT) and were divided into EGb group and ns control group randomly according to whether extract of Ginkgo Biloba leaves was injected by venous (40 mg/kg) 1h before liver grafts harvest. The third group was sham operation(SO). The animals were killed at 2, 6, 24 h after graft reperfusion respectively. Plasma samples were collected for ALT and AST tests. Fresh Liver tissues were collected to detect the expression of TNF-αmRNA and FasmRNA by RT-PCR. Parts of the liver tissues were collected in 10% formaldehyde fixed, then were made in paraffin-embeded specimens to detect rat liver histological change(HE). The serum levels of ALT in group of EGb were lower than the group of NS significantly (P <0.01) at 2, 6, 24 h after reperfusion. The serum levels of AST in group of EGb were lower than the group of NS significantly (P <0.05) at 2 h, 6 h after reperfusion. The expression of TNF-αmRNA was lower in group of EGb than the group of NS significantly(P <0.05) at 2, 6 h after reperfusion. The expression of FasmRNA was lower in group of Ginkgo Biloba extract than the group of NS significantly (P <0.05) at 2, 6, 24 h after reperfusion. Extract of Ginkgo Biloba leaves preconditioncan attenuates liver graft ischemia/reperfusion injury and affects the expression of TNF-αmRNA and FasmRNA in the liver graft.
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