Objective To make preliminary study on the relationship between saccharides and blood-tonifing effect of
Si-Wu-Tang.Methods Si-Wu-Tang was fractionated by ethanol precipitation, extraction,and macroporous adsorption resin chromatography.Activity was investigated on
irradiated mice as model of blood deficiency.Results The fraction C2(n-butanol fraction),which had previously shown activity of raising the amount of peripheral leukocyte and promoting the proliferation of hematopoietic
progenitor cell in bone marrow of irradiated mice,was separated into two fractions,fraction C2-1(
saccharide fraction) and C2-2(non-saccharide fraction),by macroporous adsorption resin chromatography.Activity experiment showed that fraction C2-1 could raise the amount of peripheral leukocyte and promote the proliferation of hematopoietic progenitor cell in bone marrow of irradiated mice,while fraction C2-2 showed only the effect of promoting the proliferation of hematopoietic progenitor cell in bone marrow.Then,according to the composition and contents of saccharides in fraction C2,D-fructose,D-glucose,and sucrose were mixed. Activity experiment showed that the saccharide mixture could promote the proliferation of hematopoietic progenitor cell in bone marrow of irradiated mice,but the effect of raising the amount of peripheral leukocyte was not significant.Conclusion To some extents,the monosaccharide and disaccharide contribute to Si-Wu-Tang's effect of improving
hematopoietic function in
Irradiated mice.
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