Search
×

Sign up

Use your Facebook account for quick registration

OR

Create a Shvoong account from scratch

Already a Member? Sign In!
×

Sign In

Sign in using your Facebook account

OR

Not a Member? Sign up!
×

Sign up

Use your Facebook account for quick registration

OR

Sign In

Sign in using your Facebook account

Shvoong Home>Medicine & Health>Determination of Sertraline in Human Plasma by LC-MS: Application to Relative Bioavailability of Dom Review

Determination of Sertraline in Human Plasma by LC-MS: Application to Relative Bioavailability of Dom

Article Review   by:TsingHua     Original Author: Chinese Pharmaceutical Journal
ª
 
OBJECTIVE To develop a HPLC-ESI-MS assay for the determination of sertraline in human plasma and to investigate the pharmacokinetics and bioequivalence of two sertraline tablets in human.METHODS After being treated with 1 mol·L~(-1) sodium hydroxide,plasma was processed by cyclohexane and compounds were separated on a reversed-phase C_(18) column with a mobile phase of methanol-10(mmol·L~(-1)) ammonium acetate buffer solution (pH 3.5)-acetonitrile(62∶28∶10).HPLC-ESI-MS was performed with the selected-ion monitoring mode.Target ions were at m/z 306.2 for sertraline and at m/z 306.1 for the internal standard.A randomized cross-over design was performed in 20 healthy volunteers.In the two study periods,a single dose of 50 mg sertraline tablet was administered to each volunteer.RESULTS The calibration curve was linear over the range of 0.5~30 μg·L~(-1).The main pharmacokinetic parameters of t_(max),ρ_(max),t_(1/2) and AUC_(0~96 h) were(5.1±1.3) h,(15.24±2.52) μg·L~(-1),(29.32±5.02) h and(347.96±41.0) μg·h·L~(-1) for the reference tablet;(4.8±0.8) h,(15.86±2.78) μg·L~(-1),(29.53±5.40) h and(338.0±29.34) μg·h·L~(-1) for the test tablet.The results of variance analysis and two one-side t-test showed that there was no significant difference between the two formulations in the AUC and ρ_(max).CONCLUSION The assay is proved to be sensitive,accurate and convenient.The two formulations are bioequivalent.
Published: March 22, 2006   
Please Rate this Review : 1 2 3 4 5
Translate Send Link Print
X

.