Object:To confirm the validaty of CO_2 supercritical fluid extraction of Radix saposhinikovise(SFE-CO_2).Methods:Take aspirin as the
positive drug.The writhing test was applied,and the times of writhing reactions of mice were observed and recorded within 15min after injected acetic acid.The variations of body temperature of rats in 3h were observed in rats with fever
induced by 2,4-dinitrophenol.Auricle tumefaction was applied to observe the degree of tumefaction in auricle induced by dimethylbenzene in mice.Results:(1)Each
dose group of SFE-CO_2 had analgesic effect,and with significant difference compared with
control group(P<0.05,low dose group;P<0.01,high dose group),but had no significant difference compared with positive control group(P>0.05),only a little better than positive control group.(2)Each dose group of SFE-CO_2 had the significant
antipyretic effect on the rats with the high body temperature induced by 2,4-dinitrophenol within 50-180min.Compared with positive control group,the antipyretic effect of SFE-CO_2 is waker,but afer 50 min and 150min,the effect of SFE-CO_2 high、middle dose group and low dose group had no remarkable difference compared with positive control group respectively(P>0.05).(3)The SFE-CO_2 high and middle dose group had anti-inflammatior activity induced by dimethylbenzene(P<0.05),but no remarkable difference was observed compared with positive control group(P>0.05).Conclusion:All of above results indicated that SFE-CO_2 has the effect of analgesic,antipyretic and anti-inflammatory.The SFE-CO_2 has the similar effect of analgesic and anti-inflammatory as aspirin,but the effect is slower in antipyretic,also,there is a relationship between dose and effect.
More abstracts about the Study on pharmacodynamics of CO_2 swpercritical fluid extraction of Radix saposhinikovise