Objective To study the effect of the compound prescription Beiejiarangan of Chinese medicine on the expression of MMP9 and
TIMPI of tublar epithelial cells stimulated by LPS in different period of time. Method To prepare drug-containing serum and inject 5% culture medium with drug-contaiming serum into tubular epithelial cells cultured in vitro and then test the
expression of MMP9and TIMPI of rats in all the experimental groups respectively by the methods of immunohistochemistry and Western blot in 24h and 48h respectively after the stimulation. Result The activity of MMP9 in cytoplasm gradually increases in 24h after stimulating tubular epithelial cells by LPS and reaches its summit at 24h and that of TTMPI also gradually increases in 24h after such stimulation,but reaches its summit at 48h.This Chinese medicine is able to lower down the expression of MMP9 in 24h as well as that of TIMPI in 48h. Conclusion The drug-containing serum prepared from the
compound prescription Biejiarangan is able to inhibit the expression of MMP9 of cells in their early stage and reduce epithelial-mesenchymal transformation caused by the damage of MMP9 to the basement of cells in its early stage while lowering down the expression of TIMPI in its later stage,thus advancing the degradation of cell epimatrix.The role of bilateral adjustment of this Chinese medicine as described above may be one of its mechanisms in the prevention and treatment of interrenal fibrosis.