Objective:To test the antivirus effects of drug 0# and 3# which are distilled from Lemon with different methods on Newcastle
disease virus and Coxsackievirus B3.Methods:Direct antivirus effect:To mix Newcastle
disease virus and Coxsackievirus B3 with drug 0# and 3# for 1 hour,then drop them into Vero cells,and culture for 72 hours;Preventive effect:To drop 0# and 3# into Vero cells for 24 hours,then to drop the mixture of Newcastle disease virus and Coxsackievirus B3 with drug 0# and 3#,and to culture for 72 hours;Therapeutic effect:To Add Newcastle the disease virus and Coxsackievirus B3 to Vero cells for 1 hour,then drop 0# and 3# into Vero cells,and to culture for 72 hours.To compare the effect of a variety of drugs on viruses through Cytopathic effect of Vero cells.Results:The drug 0# have significant difference in the direct effect on Newcastle disease virus between the test group and control group(P<0.05);The drug 3# have significant difference in the preventive effect on Coxsackievirus B3 between the test group and control group(P<0.05);The drug 0# and 3# have insignificant difference in the Therapeutic effect on Newcastle the disease virus and Coxsackievirus B3 between the test group and control group(P>0.05).Conclusion:The drug 0# have direct antivirus effect on Newcastle disease virus;The drug 3# have preventive effect on Coxsackievirus B3?