AIM To assay the contents of neuropeptide Y interleukin-2 IL-2 and interleukin-6 IL-6 in brain tissue of model rats with cerebral ischemia with radio-immunity method so as to analyze the feasibility of jiannaoning on improving memory impairment according to removing the phlegm and activating collateral flow. METHODS The experiment was completed at the Pharmacological Laboratory of Dongzhimeng Hospital Affiliated to Beijing University of Traditional Chinese Medicine from July 2000 to February 2003. Totally 152 SD rats aged 4 months of clean grade were selected in this study. Of 150 rats were randomly divided into multiple cerebral infarction group cerebral infarction in medium-sized artery group and clearing blood capillary in meninges group with 50 in each group. Rats of half gender in each group were also divided into 5 subgroups control group model group jiannaoning 11.25 3.75 and 1.25 g/kg·d groups with 10 in each group. Other 2 rats were used to collect their blood to dispense aqueous solution of thrombus. jiannaoning capsule consisted of tall gastrodia tuber ginseng leaf and bitter orange etc. provided by Wuhan Jianmin Pharmaceutical Group Limited Company batch number 000205 was dispensed suspension with 5 g/L sterilizated carboxymethylcellulose sodium. Totally 150 rats were injected with the same kind of thrombus from common carotid artery to internal carotid artery external carotid artery was ligated drugs were perfused from external carotid artery to internal carotid artery cranium was open and cortex was devascularizated. Three kinds of experimental model rats of memory disorder were developed. Rats in control group were treated with sham operation. After operation rats in control and model groups were given with 5 g/L sterilizated carboxymethylcellulose sodium according to 10 mL/kg body mass. Four weeks after medication blood was selected and plasma was separated. The contents of neuropeptide Y IL-2 and IL-6 in brain tissue of models rats in multiple cerebral infarction group cerebral infarction in medium-sized artery group and clearing blood capillary in meninges group were measured respectively with radio-immunity method. RESULTS There were 9 rats in model group 8 in jiannaoning 11.
25 g/kg·d group 9 in jiannaoning 3.75 g/kg·d group 8 in jiannaoning 1.25 g/kg·d group and 9 in control group. Totally 21 rats were excluded because of death or failing model and 129 entered the final analysis. ① Four weeks after experiment content of IL-2 in hippocampus of rats in multiple cerebral infarction group cerebral infarction in medium-sized artery group and clearing blood capillary in meninges group was significantly higher than that in model group but content of IL-6 and contents of neuropeptide Y in plasma hippocampus cerebral cortex and hypothalami of rats were significantly lower than those in model group P < 0.05-0.01. ② Content of IL-2 in hippocampus of rats in multiple cerebral infarction group cerebral infarction in medium-sized artery group and jiannaoning 11.25 g/kg·d clearing blood capillary in meninges group was significantly higher than that in model group but content of IL-6 and contents of neuropeptide Y in plasma hippocampus cerebral cortex and hypothalami of rats were significantly lower than those in model group P < 0.05-0.01. ③ Content of IL-6 and contents of neuropeptide Y in plasma hippocampus cerebral cortex and hypothalami of rats in multiple cerebral infarction group cerebral infarction in medium-sized artery group and jiannaoning 3.75 g/kg·d clearing blood capillary in meninges group were significantly lower than those in model group but content of IL-2 in hippocampus was significantly higher than that in model group P < 0.05-0.01. ④ Content of IL-6 in jiannaoning 1.25 g/kg·d clearing blood capillary in meninges group content of plasma in cerebral infarction in medium-sized artery group and content of neuropeptide Y in hypothalami of rats w