AIM The study was aimed to probe into the protective effect of ginkgo biloba extract GBE on acute lung injury in
rabbits.
GBE with the main compinents of flavonoids and lactone compounds has antistatic platelet activating factors PAF and many pharmacologic actions such as clearing oxygen free radical reducing the formation of mediator of inflammation and protecting cerebral ischemia reperfusion etc. METHODS The experiment was conducted in the Laboratory of Anesthesiology Weifang Medical College from October 2004 to June 2005. 36 healthy rabbits were randomized into
control group model group and ginkgo biloba extract group group GBE with 12 rabbits in each group. Except rabbits in the control group rest rabbits were made into acute
hemorrhagic shock models with lung injury according to Wiggers modified method Rabbits in the model group were intravenously input of lost blood and Lactated Rringer's solution of the same volume 60 minutes after hypotension and reanimated within 30 minutes Rabbits in group GBE intravenously input lost blood and Lactated Rringer's solution containing 25 mg/kg GBE extracted in the Laboratory of Anesthesiology Weifang Medical College and made into 25 g/L stable suspension by adding normal saline of the same volume following modeling so as to reanimate Rabbits in the control group thigh AtV disconnection only then intravenously input of lost blood and Lactated Rringer's solution. 1.0 mL arterial blood was taken before shock in revitalization and at the 1st and 2nd hour of treatment so as to determine the content of plasma tumor necrosis factor TNF -α with radioimmunological assay RIA. 2 hours after revitalization lung tissues in each group were got by killing the rabbits and the water ration of lung tissues was caculated humid quality- dry quality/humid quality ×100%. Positive cell rate of nuclear factor kappa-B NF-κB was calculated with immunohistohemistry and pathologically observed with light microscope. RESULTS: A total of 36 healthy experimental rabbits were involved in the analysis of results. ① The revival achievement ratio of acute hemorrhagic shock was 66.7% 8/12 rabbits and 75.0% 9/12 rabbits respectively with no significant difference P < 0.01. There were no death in the control group. ② After resuscitation the water ration of lung tissue GBE in the model group decreased more than that in the control group the enhancement in the model group was significantly higher than that in the GBE group (83.59±3.09),(76.37±3.45),(72.26±3.14);F=31.52 P < 0.01. ③Contents of TNF-α in blood plasma of rabbits in the model group and GBE group when reanimated from shock one hour and 2 hours after revitalization increased more than that in the control group and the enhancement in the model group was more significant than that in the GER group of the same time (162.38±22.33),(163.46±21.83),(74.64 ±14.29);(280.63±23.29),(259.72±17.22),(75.41±14.46);(512.44 ±23.56),(338.99±20.26),(75.98±14.26) μg/L;q=3.538-69.923,P < 0.01. ④ Two hours after revitalization NF-κB positive cell rate in lungs of rabbits in the model group and GBE group all increased(F=3615.20,P < 0.01),and that in the model group was more significant (q=62.721,P < 0.01). ⑤Lung tissues edema in lung interalveolar septum increasing and neutrophil infiltration could be seen with campeachy-eosine staining and immunohistochemistry light microscope pathological examination. The lung-tissue-injury and inflammatory cell infiltrate ICI in rabbits of GBE group were slighter. CONCLUSION GBE has some protective effect on lung injury induced by hemorrhagic shock in rabbits its mechanism may relate to the reduction of TNF-α and the inhibition of NF-κB activity in ischemic lung tissues.