AIM To study the character of
hepatic damage induced by carbon tetrachloride and analyze the effect of
compound honghua fuling
extract liquid on its prevention and cure. METHODS The experiment was completed in the Experiment Animal Center Pharmacological Laboratory and Central Laboratory of Guangdong Medical College from March 2003 to September 2005. Sixty SD rats of SPF grade were randomly divided into 6 groups normal control group model control group positive control group compound honghua fuling extract liquid 0.5 kg/L group 1 kg/L group and 2 kg/L group with 10 of either gender in each group. Three dosages of compound honghua fuling extract liquid were provided by Technological Medicament Empolder Center of Guangdong Medical College. The compound was used honghua fuling and gancao to pick-up 3 times to get the dose of 2 kg/L twice distilled water to dilute to get the dose of 0.5 kg/L and 1 kg/L. All the animals were given 600 g/L carbon tetrachloride arachis oil on the basis of 3 mL/kg subcutaneous injection once per 3 days for 7 weeks for the model of hepatic fibrosis except the control group. And the effects of compound honghua fuling extract liquid on histological change serum hyaluronic acid serum alanine aminotransferase asparagic acid transaminase total protein TP albumin Alb and weigh of visceras in hepatic tissue were observed in each dosage group. All the results were compared with colchicines to estimate the effect and character of compound honghua fuling extract liquid on preventing and curing hepatic damage induced by carbon tetrachloride. RESULTS Totally 60 rats entered the final analysis. ① Changes of liver Liver index in model group was increased 2.4 times than that in control group P < 0.01 and histopathological showed that most of hepatic cells in model group were degenerated seriously and fibrin tissue was proliferated clearly. But fiber proliferation was not observed in normal control group and hepatic fibrosis was reached 100% P < 0.05. There was not significantly different from that in crocus group. Hepatic indexes decreased 1.4 1.7 and 2.3 times as compared with those in model group respectively P < 0.05. ② Changes of serum indexes Content of hyaluronic acid in crocus group was decreased 3 times as compared with that in model group P < 0.01 2.2 2.3 and 2.6 times in compound honghua fuling extract liquid 0.5 kg/L 1 kg/L and 2 kg/L groups respectively activities of Alanine aminotransferase were decreased 1.6 1.5 and 2 times as compared with those in model group respectively activity of asparagic acid transaminase was obviously decreased P < 0.05 content of albumin was increased especially that in 0.5 kg/L compound honghua fuling extract liquid group was 1 time than that in model group P < 0.05. ③ Change of portal vein pressure The portal vein pressure in model group was higher than that in normal group but there was no significant difference and there were no significant differences among the six groups P > 0.05. CONCLUSION Compound honghua fuling extract liquid can prevent hepatic fibrosis after liver damage induced by carbon tetrachloride decrease content of transaminase and increase content of protein in rats.