AIM To explore the effect of sanchi tea bag with tea as carrier characterized by improving immunity protecting alcoholic
hepatic injury increasing anoxic endurance and relieving fatigue on blood
glucose and body mass of normal mice and model mice with type 2 diabetes. METHODS The experiment was carried in Health Food Functional Assessment Laboratory of Center for Disease Prevention and Control in Guangxi from January to March 2005. Totally 150 Kunming male mice of SPF grade were selected in this study. ① Totally 124 mice were injected with 65 mg/kg alloxan in abdominal cavity after 24-hour
fasting. Totally 100 mice were successful model in result 10-25 mmol/L fasting blood glucose. ② Test of fasting blood glucose 52 mice were treated with test of fasting blood glucose and randomly divided into 4 groups low middle and high dosage of sanchi tea bag groups and model group with 13 in each group. Low middle and high dosage of sanchi tea bag groups Mice were perfused with 375 750 and 1 500 mg/kg sanchi tea bag respectively equal to 5 10 and 20 times of recommending dose of minimal human body provided by a limited company in Yunnan sanchi saponin as the effective component volume of 10% batch number 20040801 1.5 g/bag 75 mg/kg as recommending dose of human body. Before experiment 100 g sample was dipped in 1 L 85 ℃ distilled water after filtrated it was dipped in another 1 L 85 ℃ distilled water and then was filtrated. All filtrate was concentrated to 100 mL in boiling water with reduction pressure and low temperature 60 ℃ then it was confected into 3 concentration solutions with distilled water. The volume was 0.2 mL/10 g. Model group Mice were perfused with the same volume of distilled water once a day. After 30 days the fasting blood glucose was assayed after 4 hours. The fasting blood glucose and decreasing ratio were compared between groups. Decreasing ratio of blood glucose = blood glucose before experiment-blood glucose after experiment/blood glucose before experiment × 100%. ③ Test of glucose endurance The other 48 mice were divided into 4 groups with 12 in each group according to decreasing test of fasting blood glucose. After fasting 4 hours mice were treated as the same as decreasing test of fasting blood glucose. Twenty minutes later mice in each group were perfused with 2.0 g/kg glucose to assay value of blood glucose within 0 0.5 and 2 hours respectively. The areas under blood glucose curve were compared. The area under blood sugar curve=0.25 ×value of blood glucose in zero hour + 4 × value of blood glucose in half hour + 3 × value of blood glucose in two hours. ④ Test of fasting blood glucose of normal mice 26 normal mice were divided into 2 groups high dose of sanchi tea bag group perfused with 1 500 mg/kg sanchi tea bag volume of 0.2 mL/10 g and control group perfused with the same volume of distilled water with 13 in each group. Mice were perfused once a day for 30 days then value of blood glucose 4 hours after fasting was assayed. ⑤ All mice were weighed at the beginning and the end of test. ⑥ Measurement data were compared with single factor analysis of variance. RESULTS Totally 126 mice entered result statistics. ① The body weights of mice were increased at the end of test but there were not significantly difference as compared with those before experiment P > 0.05. ② There was no difference in blood glucose between high dose of sanchi tea bag group and control group at the beginning and 30 days after experiment P > 0.05. ③ After 30-day medication the value of fasting blood glucose in each dose group were significantly lower than that in model group P < 0.05 and the decreasing ratio in high dose group was significantly higher than that in model group P < 0.05. ④ The areas under blood glucose curve of high dose group and middle dose group were significantly lower than that in model group P < 0.01 0.05. CONCLUSION ① Sanchi tea bag dose not have effect on body mass