BACKGROUND It has been shown that Qiang Chang Heji QCHJ had some protective effects in rats with experimental brain injuries and could improve some hemorheological indices. OBJECTIVE To observe effects of QCHJ on peripheral platelet in rats with experimental right brain insufficiency of blood supply. DESIGN A randomized and controlled experiment. SETTING Central Laboratory General Hospital of Chengdu Military Area Command of Chinese PLA. MATERIALS Totally 60 SD white rats either sex werghing from 150 g to 170 g were used. They were divided randomly into 3 groups sham-operation group n=8 control group n=18 and treatment group n=34. QCHJ:Chinese herbal mixture equal dosage of both Rhizoma seu Radix Notopterygii and Rhizoma Acori Graminei were decocted twice each for 30 minutes with mild fire after boiling. The two decoctions were mixed and concentrated to 1g raw herb per milliliter. METHODS The experiment was carried out in Central Laboratory General Hospital of Chengdu Military Area Command of Chinese PLA from July to September 2004. The right carotid artery CA of rats were separated ligature was done in control and treatment groups but no ligature in sham operation group. Medication po. by gastric perfusion was given since the day of operation. In treatment group 10 rats took QCHJ 10 g/kg per day for 3 days and others took 3.3 6.7 10 g/kg per day for 7 days respectively 8 rats for each dose. In control group equal volume physiological saline was given daily for 3 or 7 days. In sham operation group equal volume physiological saline was given daily for 3 days.
On the 3rd and 7th days after operation platelet count Plt mean platelet volume MPV platelet distribution width PDW and platelet-large cell ratio P-LCR of platelet were determined with a Bakeman Blood Cell Auto-analyzer. t test was used to compare the difference. MAIN OUTCOME MEASURES:Effects of QCHJ on peripheral platelet in rats with brain insufficiency of blood in brain. RESULTS ①Day 3 after operation PLt were similar between control group and sham-operation group P > 0.05 MPV PDW and P-LCR were higher in control group than that in sham operation group P < 0.01 in QCHJ treated group 10 g/kg per day MPV PDW and P-LCR were significantly lower than that in control group P < 0.01 and they were similar to that in sham operation group. ② Day 7 after operation MPV PDW and P-LCR in control group were similar to that in sham operation group.In addition P-LCR in control and QCHJ treatment group 3.3 6.7 10.0 g/kg per day were lower than that in control group on the 3rd day of operation P < 0.01. CONCLUSION Experimental brain injury induced by block blood supply may result in peripheral platelet abnormities. QCHJ has been shown to improve some of the abnormities.