AIM: To observe the effect of Chinese herb luodi shenggen on regulating blood system and immunological function of mice. METHODS: The experiment was completed in the Immunological Department of Binzhou Medical College in 2005. Luodi shenggen solution provided by Pharmacological Department of Binzhou Medical College contained 14 kinds of amino acids, such as citric acid, apple acid, succinate, fumaric acid, glyoxylic acid, pyruvic acid, grass-acetic acid, etc., quercetin-3-double-Atop-glycoside and astragulin. Totally 56 Kunming mice of clean grade were divided randomly into luodi shenggen high- and low-dosage groups, dexacortal group and saline group with 14 in each group. Mice were perfused with 800 mL/L luodi shenggen solution (0.01 mL/g) in high-dosage group, with 500 mL/L luodi shenggen solution (0.01 mL/g) in low-dosage group, with 5 mL/L dexacortal (0.01 mL/g) in dexacortal group, and the same volume saline in saline group, once a day for 15 days. Then, it was observed the effect of luodi shenggen on proliferation of spleen lymphocyte, interleukin-2 (IL-2), proliferation of bone marrow and blood system. RESULTS: Totally 56 mice entered the final analysis. ① Luodi shenggen at high concentration could promote proliferation of spleen lymphocyte, and there was significant difference from that in saline group (P < 0.
01), but luodi shenggen at low concentration did not have effect on proliferation of spleen lymphocyte. There was significant difference between dexacortal group and saline group (P < 0.05). ② Luodi shenggen at high concentration could promote IL-2 production, and there was significant difference from that in saline group (P < 0.05), but luodi shenggen at low concentration did not have effect on IL-2 production. There was no significant difference between dexacortal group and saline group (P > 0.05). ③ Luodi shenggen did not have effect on proliferation of bone marrow, and there was significant difference between dexacortal group and saline group (P < 0.01). ④ Concentration of platelet was (711.33±234.05)×109L-1 in high-dosage group and (684.20±539.53)×109L-1 in saline group, and there was significant difference between two groups (P < 0.01). Luodi shenggen at low concentration did not have effect on concentration of platelet. CONCLUSION: Chinese herb luodi shenggen can improve immunological function and accelerate blood coagulation of mice.