Aim To study the anti-tumor bioactive steroid saponins of
Paris vietnamensis(Takht.).Methods The constituents were isolated
and purified by chromatography and their structures were identified by spectral analysis and physicochemical properties.The cytotoxic bioactivities of the constituents were determined by MTT.Results Eleven compounds were obtained and identified as 3β,5α,6α-trihydroxy-7(8)-en-isospirostanol-3 -O-β-D-glucopyranosyl(1→3)<α-L-rhamnopyranosyl(1→2)>-β-D-glucopyranoside(1),whichwas named as
parisvietnaside A,25(R)diosgenin-3-O-α-L-arabinofuranosyl(1→ 4)-β-D-glucopyranoside(2),25(R)diosgenin-3- O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranos ide(3),25(R)diosgenin-3-O-α-L-arabinofurano syl(1→4)<α-L-rhamnopyranosyl(1→2)>-β-D-gluc opyranoside(4),25(R)diosgenin-3-O-β-D-gluco pyranosyl(1→3)<α-L-rhamnopyranosyl((1→)2)>- β-D-glucopyranoside(5),25(R)diosgenin-3-O-α -L-rhamnopyranosyl(1→4)α-L-rhamnopyranosyl( 1→4)<α-L-rhamnopyranosyl-(1→2)>-β-D-glucopy ranoside(6),25(R)pennogenin-3-O-α-L-arabino furanosyl(1→4)-β-D-glucopyranoside(7),25(R) pennogenin-3-O-α-L-rhamnopyranosyl((1→)2)-β -D-glucopyranoside(8),25(R)pennogenin-3-O-α -L-arabinofuranosyl(1→4)<α-L-rhamnopyranosy l-(1→2)>-β-D-glucopyranoside(9),25(R)pennog enin-3-O-β-D-glycopyranosyl(1→3)<α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(10)and 25(R)pennogenin-3-O-α-L-rhamnopyranosyl(1→4) -α-L-rhamnopyranosyl(1→4)<α-L-rhamnopyranosyl-(1→2)>-β-D-glucopyranoside(11).Someconstituents had cytotoxic bioactivities.Conclusion Compound 1 is a new spirostanol saponin,and compounds 2,3,6-11 were obtained from
Paris vietnamensis(Takht.) for the first time.Compounds 3,4,6,8 had cytotoxic bioactivities against tumor cells HepG2 and SGC-7901.