Separation and determination of donepezil hydrochloride enantiomers in plasma by capillary electroph Article Abstract
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Published: January 01, 2006
Aim To establish chiral separation method for donepezil hydrochloride(E2020) enantiomers by capillary electrophoresis (CE) and determine the two enantiomers in plasma.Methods Alkalized plasma was extracted by isopropanol-n-hexane(3∶97) and L-butefeina was used as the internal standard.Enantioresolution was achieved using 2.5% sulfated-beta-cyclodextrin as chiral selector in 25 mmol·L~(-1) triethylammonium phosphate solution(pH 2.5) on the uncoated fused-silica capillary column(70 cm×50 μm ID).The feasibility of the method to be used as quantitation of E2020 enantiomers in rabbit plasma was also investigated.Results E2020 enantiomers were separated at a baseline level under the above condition.The linearity of the response was evaluated in the concentration range from 0.1 to 5 mg·L~(-1).The linear regression analysis obtained by plotting the peak area ratio(A_(s)/A_(i)) of the analyte to the internal standard versus the concentration(C) showed excellent correlation coefficient(r=0.999 2 for R(-)-E2020,r=0.999 7 for S(+)-E2020) and the equations were A_(s)/A_(i)=0.024 2+0.289 2C and A_(s)/A_(i)=0.010 8+0.273 7C,respectively.The low limit of detection was 0.05 mg·L~(-1).The inter-and intra-day precision(RSD) were all less than 20%.Conclusion Compared with CSP by HPLC,the CE method is simple,reliable,inexpensive and suitable for studying the stereoseletive pharmacokinetics in rabbits.