Objective:To compare the protective effect on cerebral ischemia reperfusion in gerbils between pretreatment with lidocaine combined with propofol and with either lidocaine or propofol.Methods:43 Mongolian gerbils were randomly divided into five groups:(A)Control group,(B) ischemia group,(C) Lidocaine combined with Propofol pretreatment group,(D)Propofol pretreatment group,(E) Lidocaine pretreatment group.Lidocaine 30 mg/kg and propofol 100 mg/kg were given intraperitpneally(ip) 24 h before ischemia in group C.Propofol 100 mg/kg or Lidocaine 30 mg/kg was given intraperitpneally(ip) 24 h before ischemia in D or E group,respectively.There were nine gerbils in each group except seven in control group.LDH(lactate dehydrogenase) and CPK(Creatine phosphokinase)activities before decapitation were determined.The animals were then decapitated and forebrain cerebral cortex was removed to determine superoxid dismutase(SOD) and glutathione(GSH) activities and contents of malondial dehyde(MDA).One left forbrain cerebral cortex(1 mm×1 mm) was removed randomly in each group to observe the change of ultrastructure of cerebral tissue with electron microscope.
Results:In every pretreatment group MDA,LDH and CPK were significantly lower than those in ischemia group(P<0.05,P<0.01 or P<0.001)and activities of SOD and GSH were higher than those in ischemia group(P<0.05,P<0.01 or P<0.001);There were significant difference(P<0.05 or P<0.01) between Lidocaine with propofol pretreatment group and the other prtreatment groups.Compared with ischemia-reperfusion group,the ultrastructures of cerebral tissue were improved to different degree in each pretreatment group.The improvement of the ultrastructure of cerebral tissue was better in Lidocaine with Propofol group than in the other two pretreatment groups.Conclusions:Pretreatment with either lidocaine or propofol 24h before ischemia showed protective effects on cerebral ischemia reperfusion injury to different degrees in gerbils,and the protective effect was most significant in Lidocaine combined with Propofol pretreatment group.