Objective To ascertain the protective effect of oxyphenamone against myocardial ischemia and reperfusion injury in vivo.
A model of regional
myocardial ischemia-reperfusion injury(I-R) was established by(30 min) ligation of the left anterior descending coronary artery(LAD) followed by(60 min) reperfusion in hearts of anesthetized cats.The protective effects of oxyphenamone were evaluated from cardiac hemodynamics,the epicardial electrocardiogram(Epi-ECG),
myocardial energy metabolism,the content of malondialdehyde(MDA),the activities of creatine phosphokinase(CPK) and glutamic-oxaloacetic transaminase(GOT) in serum. Heart rate(HR),mean arterial pressure(MAP),left ventricle work(LVW) and systemic vascular resistance(SVR) were decreased by oxyphenamone 2.0～(8.4 mg/kg).The reaction of myocardial ischemia showing elevation of ST-segment in Epi-ECG was ameliorated.The myocardial ATP and PCr content and the ratio of ATP/Pi and PCr/Pi in the ischemic area of left ventricle were significantly lower in the control group of I-R,but this decrease was antagonized by the administration of oxyphenamone.The increases of MDA content and activities of CPK and GOT in serum induced by I-R were antagonized by oxyphenamone,resulting in the levels close to that of preischemia. Oxyphenamone can protect the hearts against myocardial ischemia and reperfusion injury in vivo.