AIM: To study the suppressive effect of glycyrrhizin (GL), a Chinese medicine, on DNFB-induced
contact hypersensitivity in mice. METHODS: BALB/c mice were divided into 5 groups according to different medication:
gl 1 (11 mg/kg) group; GL 2 (22 mg/kg) group; GL 3 (44 mg/kg) group; dexamethasone (DXM, 0.75 mg/kg) group; and normal saline group. For induction of contact hypersensitivity (CHS) to DNFB, mice were sensitized to abdomen and challenged to right
ear by epicutaneously DNFB. Each mouse was administrated intraperitoneally on day 1 to day 5 with different medication. The suppression of mice CHS by different medication were evaluated 24 hours after elicited, according to ear thickness difference, ear
weight difference and pathological change of challenged ear section. Thymus index and
spleen index were calculated to see the effect on mouse immune system to CHS. RESULTS: Compared with normal saline group, the ear thickness difference and ear weight difference were both significantly reduced in GL1, GL2, GL3 and DXM groups (P<0.01). Simultaneously histological edema and infiltration of inflammatory cell were inhibited. GL injection at three doses decreased mouse thymus weight to CHS, though mouse spleen weight were not affected, compared with NS injection. CONCLUSION: Glycyrrhizin significantly suppressed murine contact hypersensitivity.
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