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Protective effect of Extract of Ginkgo Biloba against HgCl2-induced acute renal injury in Rats
To investigate whether Extract of Ginkgo Biloba(EGb)could induce heme oxygenase-1(HO-1)in renal and attenuate
the mercuric chloride(HgCl2)-induced acute renal injury in rats. Fifty-six Wistar rats were randomly divided into four groups:groupⅠ(rats were intraperitoneally injected with EGb 20 mg/kg and 24 h later with 1 mg/kg HgCl2),groupⅡ(rats were injected with EGb 20 mg/kg + ZnPPⅨ 45 μmol/kg and then 1 mg/kg HgCl2),groupⅢ(rats were treated with equivalent volumes of saline and 1mg/kg HgCl2 at the same time)and groupⅣ(rats were given with equivalent volumes of saline and distilled water at the same time).The expression of HO-1 and its activity in renal,serum levels of creatinine(Cr),blood urea nitrogen(BUN),tissue malondialdehyde(MDA)as well as total anti-oxidative capability(TAOC)were measured.(1)EGb induced the expression of HO-1 significantly and increased the HO activity markedly.(2)Compared with groupⅣ,the serum levels of Cr and BUN and MDA content were significantly higher and renal histologic damages showed more severe,but TAOC decreased dramatically in groupⅢ;induction of HO-1 by EGb before HgCl2 injection reversed the changes above,while the protective effect of EGb against HgCl2-induced acute renal injury was suppressed by HO-1 inhibitor,Zinc protoporphyrin-Ⅸ(ZnPPⅨ). Our results indicated that EGb ameliorates HgCl2-induced acute renal injury in rats significantly by induction of HO-1.
Published: April 23, 2006
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